- Datum: –09.30
- Plats: Biomedicinskt centrum
- Föreläsare: Etsuo A. Susaki, M.D., Ph.D.
- Arrangör: Farmbio
- Kontaktperson: Anna Nilsson
CUBIC-HistoVIsion: a pipeline for three-dimensional whole-organ/body staining and imaging with single-cell resolution based on chemical properties of tissue gel
The recent development of various tissue clearing and three-dimensional (3D) imaging methods, including our CUBIC technology, enabled the comprehensive observation of whole organ/body with cellular resolution or more. Several studies tried to integrate whole-mount staining into the clearing-imaging scheme. However, due to the difficulty in penetration of stains and antibodies, they have only been applied in loose embryonic tissues or with a limited number of antibodies/stains for adult rodent tissues. To rationally identify the critical parameters for efficient penetration, we began by investigating material chemistry of fixed and delipidated biological tissue. Then, we performed a surrogate assay with an artificial material similar to tissue in order to examine multiple parameters for 3D staining. The identified parameters were integrated as a general 3D staining protocol, with which we have confirmed ~30 chemicals and antibodies used in whole adult mouse brain staining and imaging with single-cell resolution. The developed “CUBIC-HistoVIsion” pipeline for 3D histochemistry and volumetric imaging provides opportunities for multi-channel imaging of functional and structural molecules of whole adult mouse organs as well as primate organs, thus will be widely applied to life science and medical researches in future.