- Datum: –09.30
- Plats: Biomedicinskt centrum C4:2 Seminar room
- Föreläsare: Johannes Pohl, Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health
- Kontaktperson: Anna Nilsson
Using developing zebrafish embryos for toxicological evaluation of pharmaceuticals
The use of pharmaceuticals is of great importance for treatment and prevention of human and animal diseases. A considerable quantity of pharmaceutical residues will, however, end up in the aquatic environment after usage, primarily through sewage. Sewage treatment plants (STPs) are generally not designed to remove micropollutants such as pharmaceutical residues, which allows a constant release of such compounds directly into the aquatic environment. Non-target species (e.g. fish, mollusks or invertebrates) may be adversely affected since pharmaceuticals often act upon evolutionarily conserved drug targets. The zebrafish embryo toxicity (ZFET) assay is a standardized (OECD guideline no. 236) test for detecting adverse effects after exposure to chemicals or environmental samples. The ZFET assay is a robust test for detecting morphological (e.g. lethality/malformations), physiological (e.g. heart rate) and behavioral (e.g. swimming response to stressful stimuli) effects in an aquatic vertebrate. Our group has used the ZFET test to test the toxicity of wastewater from bulk drug production sites as well as environmentally relevant pharmaceuticals such as diclofenac, carbamazepine, and citalopram. Examples from our work involving ozonation as an advanced sewage treatment for the removal of pharmaceuticals will also be presented.