Bioscience seminar series
- Datum: –09.30
- Plats: Biomedicinskt centrum C4:2 Seminar room
- Föreläsare: Ario de Marco, Laboratory for Environmental and Life Sciences, University of Nova Gorica, Slovenia
- Kontaktperson: Anna Nilsson
Combining in vitro panning of nanobody pre-immune libraries with sequence (rational) engineering to get application-tailored reagents.
Antibodies possess unmet capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability and relative similarity to human sequences. The seminar will describe the general features of nanobodies, the opportunities offered
by in vitro selection of pre-immune collections, the production alternatives, the methodologies available for nanobody engineering and in silico optimization as well as some specific examples of biotechnological